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روش‌ها > فنون > فنون ایمنی‌شناختی > chromatin immunoprecipitation (en)

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chromatin immunoprecipitation (en)  

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  • Information A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of chromatin to crosslink the dna-binding proteins to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific antibodies. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.

    来源: https://lod.nal.usda.gov/nalt/336371

    创建的: 6/1/23

     (en)

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RDF/XML TURTLE JSON-LD 创建的 2023/6/1, 最后修改 2024/9/30