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methods > techniques > immunological techniques > chromatin immunoprecipitation

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void:inDataset: http://aims.fao.org/aos/agrovoc/void.ttl#Agrovoc

创建的: 2023-06-01T12:37:49

skos:notation: 5c2fc6a8

chromatin immunoprecipitation  

定义

  • Information A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of chromatin to crosslink the dna-binding proteins to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific antibodies. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.

    来源: https://lod.nal.usda.gov/nalt/336371

    创建的: 6/1/23

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RDF/XML TURTLE JSON-LD 创建的 2023/6/1, 最后修改 2024/9/30