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phương pháp > kỹ thuật > kỹ thuật miễn dịch > chromatin immunoprecipitation (en)

Terme préférentiel

chromatin immunoprecipitation (en)  

Définition

  • Information A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of chromatin to crosslink the dna-binding proteins to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific antibodies. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing. (en)

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http://aims.fao.org/aos/agrovoc/c_5c2fc6a8

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RDF/XML TURTLE JSON-LD Date de création 01/06/2023, dernière modification le 30/09/2024