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روش‌ها > فنون > فنون ایمنی‌شناختی > chromatin immunoprecipitation (en)

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chromatin immunoprecipitation (en)  

Definición

  • Information A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of chromatin to crosslink the dna-binding proteins to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific antibodies. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing. (en)

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http://aims.fao.org/aos/agrovoc/c_5c2fc6a8

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