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méthode > technique > technique immunologique > immunoprécipitation de la chromatine

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void:inDataset: http://aims.fao.org/aos/agrovoc/void.ttl#Agrovoc

Created: 2023-06-01T15:26:19

Notation: 5c2fc6a8

immunoprécipitation de la chromatine  

Definition

  • Information A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of chromatin to crosslink the dna-binding proteins to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific antibodies. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.

    Source: https://lod.nal.usda.gov/nalt/336371

    Created: 6/1/23

     (en)

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http://aims.fao.org/aos/agrovoc/c_5c2fc6a8

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RDF/XML TURTLE JSON-LD Created 6/1/23, last modified 9/30/24