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方法 > 操作方法 > 免疫技术 > 染色质免疫沉淀

Terme préférentiel

void:inDataset: http://aims.fao.org/aos/agrovoc/void.ttl#Agrovoc

Date de création: 2025-04-28T10:19:59

skos:notation: 5c2fc6a8

染色质免疫沉淀  

Définition

  • Information A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of chromatin to crosslink the dna-binding proteins to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific antibodies. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing. (en)

Concept générique

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URI

http://aims.fao.org/aos/agrovoc/c_5c2fc6a8

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RDF/XML TURTLE JSON-LD Date de création 01/06/2023, dernière modification le 28/04/2025